Book Reviews

Book Review 1Book Title: Electron microscopy methods and protocolsBook Author: Editor M.A. Nasser HajibagheriMethods in molecular biology, Volume 117. Humana Press, Totowa, New Jersey. 1999. ISBN 0-896-03640-5. Paperback, 283 pp.Book Review 2Book Title: Identifying British insects and arachnids: an annotated bibliography of key worksBook Author: Editor P.C. BarnardCambridge University Press, Cambridge. 1999. ISBN 0 521 63241 2. Hardback, 533pp

Effect of the killing method on post-mortem change in length of larvae of Thanatophilus micans (Fabricius, 1794) (Coleoptera: Silphidae) stored in 70% ethanol

It is recommended that insect larvae collected for forensic purposes should be killed using the same method as was used to create existing models for rate of development. Certain killing methods have been shown to be preferable because they cause less distortion of the specimens, but these are not always practicable in a particular case, and so a method of correcting for effect of killing method is required. Larvae of all instars of Thanatophilus micans (Fabricius 1794) (Coleoptera: Silphidae) were measured and then killed by immersion in ethanol, immersion in hot water or freezing. Samples were re-measured immediately after death, then stored in excess 70% ethanol and re-measured after 1 week and again after 4 weeks. The change in length was significantly different from zero in all samples (t = -9.07022, p < 0.001). An analysis of covariance showed that instar, killing method and storage time all had a significant effect on the change in length. The results showed that T. micans larvae have a great potential for change in length during storage but that the change is not predictable, as the magnitude and sign of the change are variable

3000 miles from home: a new Gastrosericus baobabicus Pulawski, 1995 (Hymenoptera, Larridae) distribution record highlights that the Sahel has a distinct entomofaunal signature

[from introduction] On October 30, 1953, an unidentified female wasp (Fig. 1) was collected from ‘Belet Uen, Somaliland’ (= Beledweyne, 4°44’N 45°12’E), situated in the valley of the Shebelle River, HiraanProvince,Somalia. It was deposited in the aculeate Hymenoptera collection of the Albany Museum, Grahamstown by D. Greathead prior to 1968. Apart from the words ‘Somaliland’ and ‘Desert Locust Survey’ the label is handwritten and the collector’snameis not recorded. Greathead’s sister, S. Gess (Albany Museum), deciphered the label and stated that he had worked for the Desert Locust Survey, investigating the natural enemies of locusts and had been in Somalia (then Somaliland) at that time (Murphy & Cock 2007). The specimen was sent in 2004 by F. Gess to W. Pulawski, who determined it as Gastrosericus baobabicus Pulawski, 1995. Gastrosericus species prey on spiders and a variety of small insects (Pulawski 1995), including Orthoptera (Krombein & Pulawski 1986), so it is likely that Greathead collected the specimen in connection with his interest in the insect enemies of Acridoidea (Orthoptera) (Greathead 1962)

A history of southern African research relevant to forensic entomology

Entomological forensic evidence has been used in southern Africa for decades but explicitly forensic research began in southern Africa only 26 years ago. Although applicable local research has accumulated since 1921, it is scattered in a diverse literature or unpublished. Some overseas research has also touched on local species. This review uses a historical approach to synthesize the southern African literature and to illustrate the cross-disciplinary, opportunistic nature of forensic entomology. Distinct phases of research focused on agriculture (1921-1950), medicine (1952- 1965), ecology (1968-1990) and forensics (1980-2005), but systematics spanned the entire period and tended to be ad hoc. Few scientists were involved, situated at geographically distant locations and with widely disparate research interests. The review concludes with an overview of southern African entomologists who have been involved in medico-legal investigations, and a critical evaluation of the past and future of the discipline locally

Systematic status of Plectroctena mandibularis Smith and P. conjugata Santschi (Hymenoptera: Formicidae: Ponerini)

Plectroctena mandibularis Smith is the type species of Plectroctena F. Smith. Because there has been some doubt about its distinctness from P. conjugata, several techniques were used to assess the systematic status of the two species. Most crucially, several colony series contained workers of both phenotypes, and where these series included queens or males, the distinguishing feature of these specimens was not consistently related to those of the workers. Queens, males and workers did not manifest qualitative differences between the taxa, and morphological variation was continuous between the two. The putative morphological basis (funicular index) for distinguishing workers of the taxa arose from allometric variation. Putatively diagnostic colour variation in males was related to latitude, but no simple pattern of morphological variation could be correlated with geographical distribution. Plectroctena conjugata is therefore considered a junior synonym of P. mandibularis

Book Reviews

Book Review 1Book Title: Sharks and Rays of AustraliaBook Authors: P.R. Last &amp; J.D. StevensPrinted and distributed by CSIRO, P.O. Box 89 East Melbourne. 3002 Australia, 1994. 612 pages and 84 colour plates.Book Review 2Book Title: The Ecology of Mangrove and Related Ecosystems.  Proceedings of the International Symposium held at Mombasa, Kenya 24-30 September 1990Book Authors: Edited by Victor Jaccarini &amp; Els MartensKluwer Academic Publishers, Dordrecht 1993. 272 pages. Hardbound. ISBN 0-7923-2049-2.Book Review 3Book Title: Rotifer Symposium VI. Proceedings of the Sixth International Rotifer Symposium, held in Banyoles, Spain, June 3-8, 1991Book Authors: Edited by J.J. Gilbert, E. Lubzens &amp; M.R. MiracleReprinted from Hydrobiologia, vols 255-256, as Developments in Hydrobiology 83Kluwer Academic Publishers, Dordrecht /Boston /London. 572 pagesBook Review 4Book Title: Identification Guide to the Ant Genera of the WorldBook Author: Barry BoltonHarvard University Press, 1994. 224 pages, 522 SEM photographs. ISBN 0-674-44280-

Karyology of the redfin minnows, genus Pseudobarbus Smith, 1841 (Teleostei: Cyprinidae): one of the evolutionarily tetraploid lineages of South African barbines

The karyotypes of six species of Pseudobarbus Smith, 1841, namely P. afer (Peters, 1864), P. asper (Boulenger, 1911), P. burchelli Smith, 1841, P. burgi (Boulenger, 1911), P. phlegethon (Barnard, 1938) and P. tenuis (Barnard, 1938), were examined by conventional Giemsa staining and described. All six karyotypes have 2n = 100 chromosomes, dominated by biarmed chromosomes, as does the only other member of the genus, P. quathlambae (Barnard, 1938). Sex-related intraspecific karyotype variation was not found. The shared chromosome numbers and general similarity of the karyotypes (FN = 186–192) provide a new synapomorphy to support their monophyly, which is already indicated by anatomical and mtDNA markers. Karyotype evolution within the genus has been accompanied by chromosomal inversions and centromeric shifts. Comparison of the diploid number found in Pseudobarbus with other African barbine cyprinines, which have in the region of 2n=50 and lower FNs, suggests a tetraploid evolutionary origin of the genus, possibly by allotetraploidy

Patterns and processes underlying evolutionary significant units in the Platypleura stridula L. species complex (Hemiptera: Cicadidae) in the Cape Floristic Region, South Africa

Cicadas have been shown to be useful organisms for examining the effects of distribution, plant association and geographical barriers on gene flow between populations. The cicadas of the Platypleura stridula species complex are restricted to the biologically diverse Cape Floristic Region (CFR) of South Africa. They are thus an excellent study group for elucidating the mechanisms by which hemipteran diversity is generated and maintained in the CFR. Phylogeographical analysis of this species complex using mitochondrial DNA Cytochrome Oxidase I (COI) and ribosomal 16S sequence data, coupled with preliminary morphological and acoustic data, resolves six clades, each of which has specific host-plant associations and distinct geographical ranges. The phylogeographical structure implies simultaneous or near-simultaneous radiation events, coupled with shifts in host-plant associations. When calibrated using published COI and 16S substitution rates typical for related insects, these lineages date back to the late Pliocene - early Pleistocene, coincident with vegetation change, altered drainage patterns and accelerated erosion in response to neotectonic crustal uplift and cyclic Pleistocene climate change, and glaciation-associated changes in climate and sea level